sgrnascorer.cancer.gov

sgRNA Scorer | Frederick National Laboratory

Given an input sequence or list of sequences as well as a CRISPR system with a defined spacer length and PAM sequence, sgRNA Scorer will identify putative sites and assign a predicted activity based on our new support vector machine model. In addition, an option exists to select sites with minimal off-target activity based on the CasFinder algorithm as well, enabling target sites to be selected with high predicted specificity and activity.